Table 2.
Spatial distribution of the markers used in this study in relation to the microvasculature based on the method used in Figure 1
| Normal skin | Normal colon | Colorectal adenocarcinoma | Rheumatiod arthritis | Wound healing | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| EC (PAL-E) | P (α-SMA) | EC (PAL-E) | P (α-SMA) | EC (PAL-E) | P (HMW) | EC (PAL-E) | P (α-SMA) | EC (PAL-E) | P (α-SMA) | |
| α-SMA | − | NA | − | NA | − | ++ | − | NA | − | NA |
| HMW-MAA | − | − | − | − | − | NA | − | ++ | − | ++ |
| PDGF β-receptor | − | − | − | − | − | ++ | − | ++ | − | ++ |
| a1β1 integrin | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ |
| a2β1 integrin | ++ | + | ++ | + | ++ | − | − | − | − | − |
| a3β1 integrin | ++ | + | ++ | + | ++ | − | ++ | − | ++ | − |
| a5β1 integrin | − | ++ | + | − | ++ | ++ | ++ | ++ | ++ | ++ |
| a6β1 integrin | ++ | − | ++ | − | ++ | − | ++ | − | ++ | − |
| Normal skin | Normal colon | Colorectal adenocarcinoma | Rheumatoid arthritis | Wound healing | |
|---|---|---|---|---|---|
| I | I | I (α-SMA) | I (HMW) | I (HMW) | |
| α-SMA | − | − | NA | − | − |
| HMW-MAA | − | − | − | NA | NA |
| PDGF α-receptor | − | − | − | ++ | ++ |
| a1β1 integrin | − | − | ++ | − | − |
| a2β1 integrin | + | + | + | − | − |
| a3β1 integrin | − | − | − | − | − |
| a5β1 integrin | − | − | ++ | ++ | ++ |
| a6β1 integrin | − | − | − | − | − |
Human tissues studied were normal skin, normal colon, tumour stroma formation in colorectal adenocarcinoma, pannus formation in rheumatoid arthritis and cutaneous healing wounds. Endothelial cells (EC) detected using PAL-E; pericytes (P) detected using α-SMA or HMW; interstitial fibroblast-like cells (I) detected using α-SMA or HMW.
Stained sections were analysed by computer imaging processing, as detailed in Materials and methods. The recorded percentage values represent the spatial distribution of two mAb markers in relation to each other, measured in percentage of pixels that co-localize (1 pixel equals 0.9 μm × 0.9 μm) [26, 35].
−, less than 30% co-localization; +, between 30% and 60% co-localization; ++, more than 60% co-localization.