Figure 3.

Neurons differentiated from HESC-derived NPC express MHC class I upon stimulation with IFN-γ. HESC-derived NPC were differentiated towards neurons on glass cover slips for 2 weeks and incubated or not with human recombinant IFN-γ for 3 days, before immunostaining with MHC class I combined with neuronal markers. (A) MHC class I expression by βIII-tubulin – positive neurons that were non-stimulated by IFN-γ. (B) MHC class I expression by βIII-tubulin – positive neurons that were stimulated by IFN-γ. (C) Neuritic expression of MHC class I by neurons organized in large scaffolds. (D) MHC class I expression by MAP-2 – positive neurons that were stimulated by IFN-γ (white arrows).