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. 2009 Mar 13;13(9b):3556–3569. doi: 10.1111/j.1582-4934.2009.00746.x

Figure 8.

Figure 8

Neuron quantification and expression of specific genes in the presence of cyclosporine (CsA) and dexamethasone (Dexa). (A) Quantification of the absolute number of neuronal clusters (>50 neuronal cells/cluster) in the presence or not of CsA (from 0.1 to 5 μg/ml), Dexa (from 0.05 to 5 μg/ml) or CsA + Dexa in several independent experiments (* for P < 0.05; ** for P < 0.01). (B) The total neurite outgrowth and total cell bodies areas were calculated using a specific template in the Metamorph Software. Neurite outgrowth is indicative of the total length of neuritis (arbitrary unit). The total cells body area estimate the total number of cells, including clusters (arbitrary unit) (* for P < 0.05; ** for P < 0.01). (C) Fold increase of gene expression of vimentin, PAX6, nestin and βIII-tubulin after 7 days of NPC’s culture in the presence of CsA and Dexa compared with PBS. The figure is representative of two experiments performed in triplicates (* for P < 0.05).