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. 2008 Oct 13;13(9b):3591–3615. doi: 10.1111/j.1582-4934.2008.00535.x

Figure 2.

Figure 2

Role of DNA methylation and chromatin modification in MOR gene expression. (A) – Samples were treated with TSA for 24 hrs or 5-aza-dC for 72 hrs to induce maximal effects on MOR levels in NS20Y cells. Primers for proximal promoter-derived MOR transcripts (PP-MOR) were MOR_E3-S and MOR_E4-AS (Table 1), located on exons 3 and 4, respectively, thus eliminating genomic DNA contamination in the RT-PCR reaction. (B) – Primers for distal promoter (DP)-derived MOR transcripts (DP-MOR) were D3-S and P1-AS. (C) – Combining treatment with 100 nM TSA with either 1 μM (lane 4) or 2 μM (lane 6) of 5-aza-dC enhanced the induction of PP-MOR relative to samples treated with 5-aza-dC alone (lanes 3 and 5). Primers for β-actin were used as internal controls (see Table 1).