Table 1.

List of primers used in this study

Name	Primer (5′→ 3′)	Location*	Notes
MOR-S1	CCCTCTATTCTATCGTGTGTGT	+218	Exon 1†, PP1-S‡
MOR-AS1	AGAAGAGAGGATCCAGTTGCA	+568	Exon 2, PP1-AS‡
MOR_E3-S§	CATCAAAGCACTGATCACGATTCC	+903	Exon 3, PP2-S‡
MOR_E4-AS§	TAGGGCAATGGAGCAGTTTCTGC	+1196	Exon 4, PP2-AS‡
D3-S	CCAGTCCTAATTAATTGCATATGG	−602	DP1-S‡
P1-AS	CATCCCCAAAGCGCCACTCTCTGAG	−243	DP1-AS‡
D2-S	GCACATGAAACAGGCTTCTTTCAC	−789	DP2-S‡
D6-AS	GAGGGTGGGAAGAGAGACTCTAAG	−447	DP2-AS‡
S-408	CCAATTTACACTCCTTTACACGGAAGT	−408	Real-time qPCR primer for MOR
AS-285	GGGCTGTGAGGGATCCAGAGGCTAG	−285	Real-time qPCR primer for MOR
MS-647	TTTTTAGATATGGGGATGTGGGTA	−647	MSP#
MAS-353	CTCAAAATCAACCTCCTCAATCTC	−353	MSP
MAS-180	CCAATATTCTCCTCTACTTATAACC	−180	MSP
MS-376	GAGATTGAGGAGGTTGATTTTGAG	−376	MSP
MAS-79	CTCTTCCTCTCACAACTACCCTAAAC	−79	MSP
GFAP-S1	TGGCCACCAGTAACATGCAA	+725	Exon 4, NM_010277$
GFAP-AS1	CAGTTGGCGGCGATAGTCGT	+858	Exon 5
GFAP-S2	ATGAGGAGGAAGTTCGAGAAC	+602	Exon 3/4
GFAP-AS2	CAGCAATTTCCTGTAGGTGG	+1101	Exon 6/7
Itgam-S3	CTGCCTCAGGGATCCGGAAAG	+680	Exon 7, NM_001082960$
Itgam-AS3	TGTCTGCCTCGGGGATGACATC	+829	Exon 8
Itgam-S2	TCTTGATTGATGGCTCCGGTAG	+458	Exon 6
Itgam-AS2	TTCAAAGTTGTCCACTTGGAAC	+957	Exon 9
Mtap2-S	AAGCACTGATTGGGAAGCACTC	−140	Exon 5, NM_001039934$
Mtap2-AS	CGTCGGCCATCTTTCAGATCTC	+10	Exon 6
Mtap2-S2	TTCCTCAGCTTGTCTCTAAC	−165	Exon 4
Mtap2-AS2	GCTTCAGCTGTGACTACTTG	+305	Exon 7

^*The location (starting at the 5′ end) of each primer is designated relative to +1 (as the ATG start codon).

^†The exon location of the indicated primer.

^‡Alternative names for the primers used for DP- and PP-mediated transcripts (see Fig. 4).

^§S and AS in the primer name indicate sense and antisense primers, respectively.

^#MSP: methylation-specific PCR. ‘M’ as the first capital letter of a primer name indicates a primer specific for methylated MOR DNA.

^$gb: GenBank accession number.