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. 2010 Jan 29;13(9b):3668–3679. doi: 10.1111/j.1582-4934.2009.00661.x

Figure 4.

Figure 4

5-aza-dc induces down-regulation of JAK2/STAT3/STAT5 protein levels. (A) Western blot analysis revealed decreases in JAK2 and pJAK2 protein levels for SW1116 cells treated with 5-aza-dc. In the same experiment, decreases in STAT3, pSTAT3 and pSTAT5 were also identified. The data shown are from a representative experiment and detection of GAPDH was used as a loading control. (B) Quantitative real-time PCR analysis of JAK2, STAT3 and STAT5 in SW1116 cells treated with 5-aza-dc. Results are expressed as relative expression compared to untreated cells. Each value is the mean ± S.D. of three experiments.