TABLE 1.

The number of TRAP 11-mer molecules per B. subtilis cell^a

Growth medium	No. of TRAP 11-mers/cell after growth
	Overnight	Mid-log phase
LB	340 ± 169 (22)	488 ± 193 (17)
Minimal medium
−Trp	222 ± 108 (14)
+Trp	378 ± 152 (13)

^aB. subtilis (BG2087) was grown in LB broth either overnight or to mid-log phase (A₆₀₀ of 0.8) or Vogel-Bonner minimal medium (28) in the absence and presence of 10 μg of tryptophan/ml. (−Trp and +Trp, respectively). Data are the average numbers of TRAP 11-mer molecules per B. subtilis cells ± standard deviations, are shown. The number of replicate determinations are given in parentheses. In each case, the amount of TRAP (nanograms) per microliter of treated extract was determined by comparison with the standard curve. We also determined the volume of treated extract obtained per milliliter of original cell culture and the number of cells per milliliter of culture. From these values, we calculated the amount of TRAP per cell. Using the molecular weight of 91,575 of a TRAP 11-mer, we calculated the number of molecules per cell.