HMGA2 and let-7c expression in uterine leiomyosarcoma (ULMS) in a tissue microarray (TMA)-based analysis. A. Case-matched comparison of HMGA2 and let-7c expression in 30 ULMS. Net changes of let-7c (pink dots, detected by TMA-based tissue in situ hybridization with LNA let-7c probe (see section ‘Materials and methods’) and HMGA2 (blue dots, detected by immunohistochemistry) were scored in ULMS against matched myometrium. Among those ULMS with immunoreactivity for HMGA2, loss of let-7c was more frequent (r=−0.39). B. Photomicrographs illustrating examples of tissue cores from ULMS (bottom) and matched myometrium (MM, top) in haematoxylin and eosin staining, miRNA in situ hybridization for U6 and let-7c, and immunohistochemistry for HMGA2.