Anti-proliferative effects of let-7 in ULMS cell lines SK-LMS-1 (A), SK-UT-1 (B) and SK-UT-1b (C). The cell lines were treated in three different conditions: (1) non-functional 20 nt RNA (block-iT, labelled as control, solid lines); (2) 40 pmol of exogenous let-7c (Dharmacon, Inc.) (long dash lines) and (3) exogenous let-7 inhibitor (anti-let-7, short dash lines). Cell proliferation rates were calculated (y-axis) by WST-1 staining at 24, 48 and 72 hrs after transfection. Small t-bars were standard errors.