TABLE 2.

Transformation frequencies of several plasmids in different E. faecalis strains

Plasmid	E. faecalis strain	Transformation frequencya
pAM88	JH2-2/pAM330	<10−9
pAM88It	JH2-2/pAM330	<10−9
pAM88A*	JH2-2/pAM330	3.1 × 10−7
pAM88A*	JH2-2/pAM330Δ9b	1.8 × 10−5
pAM88A*	JH2-2/pAM714	<10−9
pAM88A*	JH2-2	<10−9
pAM401	JH2-2/pAM330	1.4 × 10−6
pAM401	JH2-2/pAM330Δ9b	1.9 × 10−6
pAM401	JH2-2/pAM714	8.2 × 10−6
pAM401	JH2-2	1.0 × 10−6
pAM88	JH2-2/pAM330	<10−9
pAM88-3314	JH2-2/pAM330	2.1 × 10−7
pAM88-3316	JH2-2/pAM330	<10−9
pAM88-3318	JH2-2/pAM330	<10−9
pAM88oriV	JH2-2/pAM330	9.9 × 10−8
pAM88oriV	JH2-2	<10−9
pAM434brepA	JH2-2	5.1 × 10−5
pAM434brepAΔ9	JH2-2	<10−9
pAM88ABC	JH2-2	3.0 × 10−5
pAM88AΔ9BC	JH2-2	6.1 × 10−7
pAM401	JH2-2	1.8 × 10−6

^aThat is, the number of transformants per total number of competent cells per microgram of DNA.

^bDerived from JH2-2/pAM330Δ9/pAM88A* by spontaneous curing of pAM88A* during growth in the absence of selection for pAM88A*-encoded chloramphenicol resistance.