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. 2009 Feb 20;13(9b):3960–3972. doi: 10.1111/j.1582-4934.2009.00708.x

Figure 3.

Figure 3

Morphological changes in melanoma cells and their expression of cMyc on incubation with cardenolides. The left panel illustrates the morphological changes seen in different melanoma cell lines (annotated on the left) over 72 hrs when incubated with 100 nM UNBS1450. Human VM-21 cells are the most sensitive, whereas the mouse B16F10 model is completely resistant to the treatment. The right panel corresponds to Western blot analysis of cMyc expression levels in each cell line after 15 hrs of exposure to four different cardenolides (UNBS: UNBS1450, OUA: ouabaïn, DIGO: digoxin and DIG I: digitoxin) at 10 and 100 nM. Although cMyc is down-regulated after UNBS1450 treatment (100 nM) in the VM-21, VM-24 and C32 responding cell lines, this is not the case in the poorly responding or non-responding SKMEL-28 and B16F10 cells. Tubulin or histone H3 blots were used as the loading controls.