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. 2009 Apr 2;13(9b):4023–4033. doi: 10.1111/j.1582-4934.2009.00723.x

Figure 4.

Figure 4

Effects of EpCAMxCD3 on the production of effector cytokines by pre-activated lymphocytes in 3D tumour reconstructs. (A) A schematic graph depicting the composition of collagen gel 3D tumour reconstructs. The production of IFN-γ (B) and TNF-α (C) in collagen gel 3D tumour reconstructs consisting of BxPC-3 carcinoma cells, pre-activated human lymphocytes and fibroblasts was analyzed by ELISA. EpCAMxCD3, control parental anti-human EpCAM mAb, or irrelevant bsAb CD19xCD3, respectively, were added to 3D tumour reconstructs (n= 4 per group) after the collagen gel polymerization. Supernatants from 3D tumour reconstructs were collected after 24 or 72 hrs of incubation of mixed cell cultures. *P < 0.05.