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. 2009 Apr 2;13(9b):4023–4033. doi: 10.1111/j.1582-4934.2009.00723.x

Figure 5.

Figure 5

Effects of EpCAMxCD3 on the production of effector cytokines by non-stimulated PBMCs in 3D tumour reconstructs. The ability of EpCAMxCD3 to induce the production of IFN-γ (A), TNF-α (B) and IL-2 (C) by non-stimulated PBMCs was analyzed in 3D tumour reconstructs with BxPC-3 carcinoma cells. Non-stimulated PBMCs were incubated with carcinoma cells and fibroblasts in 3D collagen gels for 24 or 72 hrs (n= 4 per group). EpCAMxCD3, control parental anti-human EpCAM mAb, irrelevant bsAb CD19xCD3 or control bivalent anti-CD3 mAb, respectively, were added to 3D tumour reconstructs after the collagen gel polymerization. Cytokine levels in supernatants from 3D collagen gels were analyzed by ELISA.