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. 2009 Apr 2;13(9b):4023–4033. doi: 10.1111/j.1582-4934.2009.00723.x

Figure 6.

Figure 6

Effects of EpCAMxCD3 on the production of inhibitory cytokines by non-stimulated PBMCs in 3D tumour reconstructs. The ability of EpCAMxCD3 to induce the production of IL-10 (A) and TGF-β1 (B) by non-stimulated PBMCs was analyzed in 3D tumour reconstructs with BxPC-3 cells. Non-stimulated PBMCs were incubated with carcinoma cells and fibroblasts in 3D collagen gels for 24 or 72 hrs (n= 4 per group). EpCAMxCD3, control parental anti-human EpCAM mAb, irrelevant bsAb CD19xCD3 or control bivalent anti-CD3 mAb, respectively, were added to 3D tumour reconstructs after the collagen gel polymerization. Cytokine levels in supernatants from 3D collagen gels were analyzed by ELISA.