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. 2009 Nov 19;13(9b):4061–4076. doi: 10.1111/j.1582-4934.2009.00975.x

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© 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Analysis of apoptosis and necrosis in colons of I/R injury rats. (A) Representative TUNEL stains in colon tissues obtained from sham-operated and I/R rats. Apoptotic cells were shown as bright green fluorescence staining. (B) Detection of necrosis with PI staining. The DAPI (blue) and PI (red) fluorescence were shown. Quantitative analysis of TUNEL⁺ cells (C) and necrotic cells (D) in the colon at various time-points of reperfusion after 1 hrs ischaemia. White arrows depict TUNEL⁺ cells (A) and necrotic cells (B). Bars = 10 μm.

Analysis of apoptosis and necrosis in colons of I/R injury rats. (A) Representative TUNEL stains in colon tissues obtained from sham-operated and I/R rats. Apoptotic cells were shown as bright green fluorescence staining. (B) Detection of necrosis with PI staining. The DAPI (blue) and PI (red) fluorescence were shown. Quantitative analysis of TUNEL⁺ cells (C) and necrotic cells (D) in the colon at various time-points of reperfusion after 1 hrs ischaemia. White arrows depict TUNEL⁺ cells (A) and necrotic cells (B). Bars = 10 μm.