Figure 6.

Western blotting analysis of the lipid raft marker protein caveolin-1 and TJ protein Occludin, ZO-1 and claudins 1, 3 and 5. Tissues were homogenized and subjected to discontinuous sucrose density gradient ultracentrifugation. The resulted fractions were analysed by immunoblotting. Blots were probed with antibodies and analysed quantitatively by densitometry with Quantity One 1-D analysis software. The proteins examined were found to translocate from TX-100 insoluble fractions to TX-100 soluble fractions. The blots shown are representative of three experiments. (A, B) Caveolin-1; (C, D) Occludin; (E, F) ZO-1; (G, H) claudin-1; (I, J) claudin-3; (K, L) claudin-5. Data presented were mean ± S.E.M. (***P < 0.001).