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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Virology. 2015 May 15;483:209–217. doi: 10.1016/j.virol.2015.04.014

Fig. 2.

Fig. 2

CPXV219 is not essential for virus replication. (A) One step growth curve. HeLa cells were infected with 3 PFU/cell of the parent CPXV strain Brighton (CPX-BR) and CPXV 219delGFP, 219Rev and 219Stop and the cells plus media were collected at the indicated times. The cells were lysed by repeated freeze-thawing and the virus titers were determined by plaque assay on BS-C-1 cells. (B) Plaque formation. BS-C-1 cells were infected with the indicted viruses, overlaid with semi-solid medium and stained with crystal violet after 72 h.