Table 2.

Confirmed lipid-droplet proteins of early Drosophila embryos

Protein	Evidence for droplet localization	Amount
Kinesin	Western analysis [76] Colocalization on purified droplets [58] Mutants and antibody injection alter droplet motility [76]	Minor
Dynein	Western analysis [76, 87] Colocalization in disrupted embryos [77] Colocalization on purified droplets [58] Mutants alter droplet motility [77, 84] Mutants for known dynein cofactors alter motility [83, 84]	Minor
Dynactin	Colocalization on purified droplets [58] Mutant alters droplet motility [84]	Minor
LSD-2	Western analysis [87] Colocalization in disrupted embryos [87] Centrifuged embryos [87] Mutants alter droplet motility [87]	Likely exclusively on droplets
BicD	Western analysis [83] Mutants alter droplet motility [83]	Minor
Klar	Colocalization in intact embryos [105] Centrifuged embryos [56, 76, 105] Mutants alter droplet motility [56, 57, 105] Mutants that alter droplet distribution alter Klar distribution accordingly [76, 105]	Large fraction
H2A	Western analysis [15, 16] Centrifuged embryos [15, 16]	Large fraction in early embryos
H2B	Western analysis [15, 16] Centrifuged embryos [15, 16]	Large fraction in early embryos
H2Av	Western analysis [16] Centrifuged embryos [15, 16, 75] Colocalization in intact embryos [15] Mutants that alter droplet distribution alter H2Av distribution accordingly [15]	Large fraction in early embryos
Jabba	Western analysis [16] Centrifuged embryos [16] Colocalization in intact embryos [16] Mutants abolish histone sequestration on droplets [16] Mutants that alter droplet distribution alter Jabba distribution accordingly [16]	Large fraction, possibly exclusively on droplets

Amount refers to the fraction of the protein detected on lipid droplets compared to the total levels of the protein in the embryo.