FIG. 1.

Physical maps of the lepB and lepA genes encoding the lysyl endopeptidases from Lysobacter sp. strain IB-9374 (A) and the expression plasmid pGEX-lepB (B). (A) pLBP5 and pLBS8 are the insert DNAs subcloned into pUC118. The arrows indicate the sizes, directions, and locations of the lepB and lepA genes. The lepB gene is depicted below. The restriction site designations are as follows: B, BamHI; E, EcoRI; P, PstI; Sm, SmaI; Sp, SphI. (B) The partial lepB gene containing the propeptide and mature enzyme regions was amplified by PCR, and the DNA fragment was inserted into BamHI and EcoRI sites downstream of the GST gene in pGEX-6P-1. Ptac is the tac promoter.