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Effects of temperature on the amidolytic activities of LepB and LepA. (A) Optimum temperature. The amidolytic activities of LepB (solid circles) and LepA (open circles) were measured at various temperatures, as indicated. (B) Heat stability. After 1.1 μg of LepB or LepA was incubated at various temperatures, as indicated, for 30 min, the residual amidolytic activities against Bz-Lys-pNA were measured.
