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Effects of various concentrations of urea (A), guanidine-HCl (B), and SDS (C) on the amidolytic activity of LepB. After 1.1 μg of the purified LepB (solid circles) or LepA (open circles) was incubated at 30°C for 20 min in the presence of various concentrations of urea, guanidine-HCl, or SDS, as indicated, the amidolytic activities against Bz-Lys-pNA were measured in the presence of the same denaturant at a given concentration.
