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Purification of recombinant LepB
| Step | Protein (mg) | Total activity (U) | Sp act (U/mg of protein) | Purification (n-fold) | Recovery (%) |
|---|---|---|---|---|---|
| Refolded enzyme | 102 | 4.8 | 0.047 | 1 | 100 |
| DEAE-Toyopearl | 26.2 | 4.5 | 0.17 | 4 | 94 |
| AH-Sepharose 4B | 0.81 | 3.0 | 3.7 | 79 | 63 |
| Sephacryl S-200 | 0.54 | 2.3 | 4.3 | 91 | 48 |
