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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Cell Physiol. 2015 Nov;230(11):2683–2694. doi: 10.1002/jcp.24991

Figure 2. BRG1 or BRM reduction in MDA-MB-231 metastatic breast cancer cells reduced colony formation in culture and tumor growth in vivo.

Figure 2

(A) Western blot indicating BRG1 and BRM levels in MDA-MB-231 cells inducibly expressing a control (Scram) shRNA or shRNA targeting BRG1 or BRM. GAPDH levels were monitored as a loading control. DOX; doxycycline. (B) Colony forming assay demonstrates that BRG1 or BRM knockdown reduces the ability of MDA-MB-231 cells to form colonies when plated at low density. (C) Quantification of colony forming assay. Quantification is the average from 3 independent experiments; error bars, SD. ***P<0.001. (D) BRG1 or BRM knockdown cells formed fewer and smaller tumors after implantation in the mammary fat pad of SCID/NCr mice. Each bar represents averaged results, n=12; error bars, SD. ***P<0.001. (E) Images of representative tumors.