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. Author manuscript; available in PMC: 2016 Sep 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 May 21;412:226–238. doi: 10.1016/j.mce.2015.05.008

Figure 5.

Figure 5

Knockdown of Xlr5c-like expression by Xlr5c-like siRNA and its effect on Granulosa cells isolated from rat preovulatory ovaries (48 h post-PMSG) were transfected with negative control siRNA (NC siRNA) or Xlr5c-like siRNA, and stimulated with FSK+PMA for 12 h or 20 h. A) The level of mRNA for Xlr5c-like was measured by real-time PCR and normalized to the L32 value in each sample. B) Concentration of progesterone was measured in the spent media of granulosa cells transfected with siRNAs and cultured with FSK+PMA for 12 h or 20 h. Experiments were repeated 4 times, each with different granulosa cell sample (mean ± SEM). Bars with no common superscripts are significantly different (p< 0.05).