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. Author manuscript; available in PMC: 2016 Sep 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 May 21;412:226–238. doi: 10.1016/j.mce.2015.05.008

Figure 7.

Figure 7

The expression of flag-tagged rat Xlr5c-like in granulosa cells. Granulosa cells isolated from rat preovulatory ovaries (48 h post-PMSG) were infected with an adenovirus containing null vector (Ad-ϕ) or expressing flag-tagged Xlr5c-like (Ad-Xlr5c-like), and stimulated with FSK+PMA for 14 h. A) Flag-tagged Xlr5c-like protein was immunostained with monoclonal anti-flag antibody (M2), followed by TRITC-conjugate-goat anti-mouse IgG (red staining, b & e). The cells infected with Ad-Xlr5c-like also express hrGFP (green staining, c). Note that hrGFP freely defuses to the nucleus, staining for both the nucleus and cytoplasm. Nuclei were stained by DAPI (blue staining, a & d). Panel f represents the merged image of d and e. Scale bar, 20 μm for all the images. B) Cell lysates from adenovirus-infected cells were subjected to Western blots using anti-flag antibody (M2). β-Actin was used as a loading control.