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ASO-PCR primer sequences and predicted product sizes.
| Lineage | Primer | Primer sequencesa (5′-3′) | PCR product size (bp) |
|---|---|---|---|
| 1 | actA1-f | AATAACAACAGTGAACAAAGC | 373 |
| actA1-r | TATCACGTACCCATTTACC | ||
| 2 | plcB2-f | TTGTGATGAATACTTACAAAC | 564 |
| plcB2-r | TTTGCTACCATGTCTTCC | ||
| 3 | actA3-f | CGGCGAACCATACAACAT | 277 |
| plcB3-r | TGTGGTAATTTGCTGTCG |
Underlined nucleotides are specific to the L. monocytogenes lineage listed in the first column.
