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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Neurochem. 2015 May 25;134(4):629–641. doi: 10.1111/jnc.13160

Figure 5.

Figure 5

Acute ethanol administration results in phosphorylation and inactivation of STEP61 and increased synaptic localization of PTPα in DMS. C57BL/6 mice (2–3 months) were injected with ethanol (EtOH) acutely (2 g/kg, i.p.) for 15 min. Mice brain regions DMS (a), DLS (b) and NAc (c) were dissected and crude synaptic membrane fractions were isolated and used for western blotting with phospho-specific and pan antibodies. Quantification of phosphorylation levels were normalized to total protein levels and then to β-actin as a loading control. All data were expressed as mean ± SEM (*p < 0.05, Student’s t-test; n = 5).