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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Neurochem. 2015 May 25;134(4):629–641. doi: 10.1111/jnc.13160

Figure 6.

Figure 6

STEP61 regulates translocation of PTPα into lipid rafts fraction in DMS upon acute ethanol administration. (a, b) WT (C57BL/6, 2–3 months) and STEP KO (C57BL/6 background) mice were injected with ethanol (EtOH) acutely (2 g/kg, i.p.) for 15 min. DMS was dissected out and subjected to sucrose gradient fractionation to obtain lipid rafts and non-rafts fraction. Distribution of PTPα, STEP61 and Fyn in each fraction were probed with specific antibodies. Flotillin-1 (Flot-1) and transferrin receptor (TfR) were used as markers. (c) Distribution of PTPα, STEP61 and Fyn in rafts fraction (fractions 3 and 4) and non-rafts fraction (fractions 10–12) were probed with specific antibodies. Representative results were shown from 5 mice each group (n = 5). Data were expressed as mean ± SEM (**p < 0.01, ***p < 0.001, two-way ANOVA with post hoc Tukey’s test; n = 5).