FIG. 1.
(a) Structural model for the full-length Tsr dimer based on structures of the periplasmic and cytoplasmic domains, adapted from the work of Kim et al. (14). (b and c) Projection images of thin (70-nm) sections of fixed specimens of either wild-type E. coli (b) or an E. coli Tsr-overexpressing strain (HCB721/pHSe5.tsrQEQE) induced with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) (c). Scale bars = 0.5 μm. (d) Higher magnification of cells overproducing Tsr. Scale bar = 50 nm. The dark spots in the images are from 10-nm gold-conjugated protein A used to locate Tsr.