TABLE 2.
Antifeeding effects of the cloned antifeeding-encoding region (pAF6), its mutated derivatives, pADAP-based subclones, and pADAP deletion derivatives
| Construct | % Feedinga | One tailed t-testb
|
|
|---|---|---|---|
| df | P | ||
| S. entomophila A1MO2 | 0 | ||
| Control (no bacteria) | 100.0 | ||
| pMH52 | 100.0 | ||
| pMH53 | 100.0 | ||
| pMH54 | 100.0 | ||
| pAD32ΔBglII | 100.0 | ||
| S. entomophila(pAF6) | 0 | ||
| E. coli XL1-Blue(pAF6) | 0 | ||
| pADK93XbaIΔ14XbaI | 100.0 | ||
| pADC20XbaIΔStuI | 100.0 | ||
| pADK93XbaIΔStuI | 100.0 | ||
| pADS93XbaIΔStuIΔBglIIKn | 0 | ||
| Mutated derivatives of pAF6 | |||
| pAF6-1 | 100.0 | 0 | |
| pAF6-2 | 0.0 | 1 | NV |
| pAF6-3 | 49.0 | 2 | 0.095 |
| pAF6-4 | 0.0 | 0 | |
| pAF6-5 | 16.7 | 1 | 0.25 |
| pAF6-6 | 0.0 | 0 | |
| pAF6-7 | 0.0 | 0 | |
| pAF6-8 | 0.0 | 0 | |
| pAF6-9 | 91.7 | 0 | |
| pAF6-10 | 82.5 | 1 | 0.029* |
| pAF6-11 | 72.8 | 2 | 0.017* |
| pAF6-12 | 83.3 | 2 | 0.007** |
| pAF6-13 | 88.9 | 2 | 0.000** |
| pAF6-14 | 100.0 | 1 | NV |
| pAF6-15 | 0.0 | 0 | |
| pAF6-16 | 100.0 | 1 | NV |
| pAF6-17 | 76.4 | 2 | 0.006** |
| pAF6-18 | 0.0 | 0 | |
| pAF6-19 | 0.0 | 1 | NV |
| pAF6-20 | 100.0 | 0 | |
| pAF6-21 | 0.0 | 1 | NV |
| pAF6-22 | 0.0 | 0 | |
| pAF6-23 | 85.2 | 3 | 0.003** |
| pAF6-24 | 0.0 | 1 | NV |
| pAF6-25 | 0.0 | 0 | |
| pAF6-26 | 0.0 | 1 | NV |
| pAF6-29 | 0.0 | ||
| pAF6-30 | 4.2 | 1 | 0.20 |
| pAF6-31 | 86.3 | 4 | 0.000** |
| pAF6-32 | 0.0 | 0 | |
a
Percentage of larvae treated with bacteria feeding on fresh carrot in the period 3 to 6 days after treatment.
b
One-tailed t test for comparison of feeding by larvae treated with mutant derivatives of pAF6 with feeding by larvae treated with nonmutated pAF6 in the same batch assay. df, degrees of freedom; P, probability of getting the result by chance; *, significance < 0.05; **, significance < 0.01; NV, no variation.