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. 2004 Aug;186(15):5087–5092. doi: 10.1128/JB.186.15.5087-5092.2004

FIG. 6.

FIG. 6.

New biofilm matrix accumulates on top of old. Columns: GFP, fluorescent bacteria; Texas red, labeled biofilm matrix; Merge, both images. Rows: top, nematodes immediately after 30 min on Y. pseudotuberculosis-GFP incubation and WGA-TR labeling; bottom, nematodes incubated for 30 min on Y. pseudotuberculosis-GFP, labeled with WGA-TR, and then transferred to nonfluorescent Y. pseudotuberculosis for 20 h. Panels: A and D, combined DIC and GFP images; B and E, combined DIC and TR images; C and F, combined DIC, GFP, and Texas red images. Arrows indicate unlabeled new biofilm matrix.