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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2015 May 23;1851(9):1227–1239. doi: 10.1016/j.bbalip.2015.05.003

Fig. 6. The observed changes in Coq polypeptide levels with temperature do not correspond to alterations in COQ RNA content.

Fig. 6

The total RNA of W3031B (WT) and W303ΔCOQ9 harboring the temperature-sensitive plasmid TS19 (Δ9:TS19) were extracted after yeast were grown for 18.5 hours at 25°C or 37°C. RNA were also extracted from the null control strains W303ΔCOQ9 (Δ9), W303ΔCOQ7 (Δ7), and W303ΔCOQ4 (Δ4) after yeast were grown overnight at 30 °C. Aliquots of RNA (5 μg) were separated by 1.2% agarose gel and analyzed by Northern blot. Hybridizations were performed with probes against the designated RNA: COQ4, COQ5, COQ6, COQ7, COQ9 and SCR1. Northern blot assay signals were quantified with the Quantity One software from the Bio-Rad FX Plus Phosphorimaging System. The quantified signals of COQ4, COQ5, COQ6, COQ7, and COQ9 were each normalized by the signals of SCR1. Normalized values were then compared to WT at 25°C to get the relative percentage. Each bar represents a total of two measurements from two independent samples (n=2). Significant changes in the amounts of mRNA at different temperatures were determined with the Student’s two-tailed t-test. The *symbols represent mRNA in samples at 37 °C compared to mRNA at 25 °C; *, p<0.05, **, p<0.01, ***, p<0.001.