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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2015 May 23;1851(9):1227–1239. doi: 10.1016/j.bbalip.2015.05.003

Table 4.

Primer sequences (site-directed mutagenesis of S. cerevisiae COQ9)

Primer name Sequence
E55Gf 5′-AGAGAAACCGTGCCCG GGAACAAAC-3′
E55Gr 5′-GTTTGTTCCCGGGCACGGTTTCTCT-3′
R107Gf 5′-GGGTTGATTCCTTCAGTTAAACGATACCCTTTATCTACC-3′
R107Gr 5′-GGTAGATAAAGGGTATCGTTTAACTGAAGGAATCAACCC-3′
Q256Lf 5′-CCCCTAACTAATAGAGATTTGATTAAATTTA CCGTAGACA-3′
Q256Lr 5′-TGTCTACGGTAAATTTAATCAAATCTC TATTAGTTAGGGG-3′
a-12gf 5′-GAGATAACAGAGTCTTTACCGCATTATAAATC-3′
a-12gr 5′-GATTTATAATGCGGTAAAGACTCTGTTATCTC-3′
a-93gf 5′-GCAATAACAATAGTAAGAAACGATAATACGGGG-3′
a-93gr 5′-CCCCGTATTATCGTTTCTTACTATTGTTATTGC-3′