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X-band EPR spectra of M2TMC spin-labeled at position 45 in DLPC/DLPG 4:1, DMPC/DMPG 4:1 and POPC/POPG 4:1. Peptide lipid molar ratio 1:200. Dilute-labeled spectra are shown in grey and fully labeled spectra are shown in black. Addition of M2TMC C50S was used for dilute-labeled spectra. Dilute-labeled samples have one or less spin label per tetramer. All spectra have been normalized to the same number of spins.
