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. Author manuscript; available in PMC: 2016 Sep 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 May 29;412:73–84. doi: 10.1016/j.mce.2015.05.030

Figure 4. Effect of PMA, ATP and VEGF-165 and the inhibition by U0126 on Cx43 phosphorylation.

Figure 4

NP-UAEC and P-UAEC (left and right graphs, respectively) were pretreated for 20 min with 10 uM U0126, followed by a 30-min treatment with 10 nM PMA, 100 uM ATP or 10 ng/ml VEGF. Results were normalized to Hsp90 protein and expressed as fold change over unstimulated control. Phospho-specific western blot analyses were performed, and representative blots are shown. Values shown are Means ± S.E.M. of 5–6 independent experiments (* P<0.05 compared with control; + P<0.05 compared with same agonist treatment; # P<0.05 compared with NP-UAEC). A. Response of Ser-279/282 Cx43. B. Response of Tyr-265 Cx43. C. Response of the 42 kDa band of s368 Cx43.