Table 3B.
The effect of anti-G protein mAb 131-2G and anti-F protein 143-6C treatment on Th2 type cytokine levels in r19F RSV infected mice.
| Untreated | mAb 131-2G treated | mAb 143-6C treated | ||
|---|---|---|---|---|
| Days (pi) | Th2 cytokines | pg/gram of lung | pg/gram of lung | pg/gram of lung |
| 4 | IL-4 | 15.21±3.19 | 14.35±2.61 | 14.00±1.77 |
| IL-5 | 10.65±0.32 | 4.67±0.77* | 9.84±0.47 | |
| IL-13 | ND | ND | ND | |
| 5 | IL-4 | 45.14±15.54 | 12.34±3.23** | 34.56±12.43 |
| IL-5 | 14.30±1.16 | 2.34±1.56** | 6.71±2.49 | |
| IL-13 | ND | ND | ND | |
| 6 | IL-4 | 30.20±5.91 | 12.11±3.83* | 24.34±9.20 |
| IL-5 | 4.55±2.38 | 1.68±0.71 | 3.01±1.45 | |
| IL-13 | 12.86±1.98 | 4.86±2.01* | 11.98±1.68 | |
| 7 | IL-4 | 19.74±3.58 | 11.33±1.60 | 11.26±4.15 |
| IL-5 | 1.82±0.62 | 0.92±0.41 | 2.00±1.16 | |
| IL-13 | 16.84±3.32 | 4.21±1.28** | 10.86±2.49 | |
| 8 | IL-4 | 4.68±2.75 | 1.25±1.09 | 2.99±2.18 |
| IL-5 | ND | ND | ND | |
| IL-13 | 28.34±5.36 | ND** | 5.68±1.42** | |
BALB/c mice were infected with r19F (1×106 TCID50) on day 0 and administered 300 μg of anti-G (131-2G) or anti-F (143-6C) protein mAbs on day 3 pi (n=5 mice/group). The lungs were harvested on the indicated days in the Table 3B. The lung homogenates were tested by luminex multiplex assay. Data are means ± SEM.
*
(p≤0.05, ANOVA)
**
(p≤0.001, ANOVA) indicates significant changes in cytokine concentrations for untreated compared to mice receiving the indicated treatments. Results are representative of two independent experiments.