Table 1.
Monoclonal antibodies reactive to antigens in human extrahepatic biliary cells (EHBCs)
| Antibody | Immunofluorescence staining patterns in gallbladder and cystic duct | Antibody isotype (Mouse) | Frequency of positive cells
|
|
|---|---|---|---|---|
| Gallbladder | Cystic duct | |||
| GB1* | Subset of mucosal epithelium | IgG | 2% | 1% |
| GB2* | All Mucosal epithelium with apical bias | IgG | 8% | 3% |
| GB3* | Subset in the muscularis layer; subset of peribiliary glands | IgM | 6% | 6% |
| GB4* | All Mucosal epithelium + peribiliary glands and surrounding connective tissues | IgG | 5% | 2% |
| GB5* | Mucosal epithelium with basal bias | IgG | 5% | 2% |
| GB6* | All Mucosal epithelium + subset of lamina propria + peribiliary glands and surrounding connective tissues | IgM | 80% | 80% |
| GB7* | Mucosal epithelium with subcellular localization | IgM | 1% | <1% |
| GB8* | Mucosal epithelium with subcellular localization | IgM | 2% | 1% |
| GB9 | Subset of mucosal epithelium | IgM | 6% | 2% |
| GB10 | Subset of mucosal epithelium with apical bias + peribiliary glands | IgG | 3% | 2% |
| GB11 | Mucosal epithelium | IgM | 7% | 2% |
| GB12 | Mucosal epithelium with apical bias | IgG | 10% | 3% |
| GB13 | Mucosal epithelium | IgG | 9% | 3% |
| GB14 | Secreted/Luminal | N.D. | 0 | 0 |
| GB15 | Circumference of large blood vessel walls | IgM | <1% | <1% |
| GB16 | Mucosal epithelium, peribiliary glands | IgG | 4% | 2% |
| GB17 | Subset of mucosal epithelium with apical bias | IgM | 2% | 1% |
| GB18 | Mucosal epithelium with apical bias; peribiliary glands | IgG | 7% | 2% |
| GB19 | Subset of mucosal epithelium with apical bias | N.D. | 0 | 0 |
| GB20 | Mucosal epithelium and subsets in the muscularis layer | IgG | 8% | 4% |
Antibodies marked with asterisks (*) were selected for dual labeling fluorescence-activated cell sorting to subdivide adult human EHBCs. GB=human gallbladder. N.D. not determined