Figure 1. Electrophysiological properties of layer V prelimbic prefrontal cortical (PL-PFC) pyramidal neurons from wildtype (Wt) and Pvalb/Gad1 transgenic (Tg) mice.<.

br>(A) Representative sIPSC traces for Wt (black) and Tg (grey) PL-PFC neurons. Calibration bars represent 20 pA and 100 ms. (B) Cumulative probability distributions of sIPSC inter-event interval (left) and amplitude (right) recorded from Wt (black) and Tg (grey) PL-PFC neurons. Insets show that average sIPSC frequency (Hz), but not amplitude (pA) is significantly reduced in Tg as compared to Wt PL-PFC pyramidal neurons (p = 0.0006 and p = 0.3939, respectively; n=13/condition). (C–I) Reduction of GAD1 does not change the kinetic IPSC properties or the intrinsic excitability of layer V PL-PFC pyramidal neurons. No significant changes were observed in the average (C) sIPSC rise (ms; p=0.9297, n=13/condition ) or (D) decay time (ms; p=0.6919, n=13/condition), (E) resting membrane potential (mV; p=0.3141, n=10–11), (F) input resistance (MΩ; p=0.7469, n=11–12), (G) action potential firing frequency (Hz; p>0.05 n=11–12), (H) rheobase current (pA; p=0.8465, n=11–12), and (I) latency to first action potential (ms; p=0.6713, n=11–12). ***p<0.001; Number of cells per experimental conditions is also indicated in bar graphs.

N.S. denotes not statistically significant. Error bars represent standard error of means (SEM).