Fig. 6.

Quantitative analyses of the effect of chemical stimulation on cultured neural networks. Four different chemicals: (a) NMDA, (b) AMPA, (c) GABA, and (d) bicuculline was examined. Quantitative features of neural activity, such as mean firing rate (MFR), inter-spike interval (ISI), spike amplitude (amplitude), and the ratio of active cells that showed calcium signals among all detected ROIs, were measured ($\text{mean}\pm \mathrm{SEM}$; $n=5$; $***p<0.001$; ns: not significant).