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. 2015 Aug;146(2):183–192. doi: 10.1085/jgp.201511388

Table 3.

Halftimes (t1/2, in seconds) of LFN translocation at ΔpH = 0.65 and 1.0

Channel t1/2, ΔpH = 0.65 t1/2, ΔpH = 1
WT (PA63)7 23 ± 0.26 2.7 ± 0.55
(PA63E302S)7 20 ± 4.4 2.1 ± 0.49
(PA63E308S)7 22 ± 2.5 4.0 ± 0.83
(PA63D315S)7 n/a n/a
(PA63D335S)7 50 ± 7.2 4.9 ± 0.96
(PA63E343S)7 43 ± 6.0 2.5 ± 0.42

Halftimes (t1/2, in seconds) of LFN translocation at ΔpH = 0.65 and 1.0. Each halftime represents the mean t1/2 of three experiments. Note that for the (PA63D315S)7 channel, translocation was too slow to observe t1/2. n/a, not applicable.