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. 2015 Aug;146(2):161–172. doi: 10.1085/jgp.201511359

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© 2015 Combs and Lu

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Effect of SMase D on C-type inactivation of K_V1.3 channels in human T lymphocytes. (A and B) K_V1.3 currents elicited by stepping membrane voltage at 30-s intervals from the −100-mV holding potential to a first pulse between −90 and −10 mV in 10-mV increments followed by a second test pulse to 0 mV, before (A) and after (B) the addition of SMase D to the bath solution (0.25 µg/ml). Current traces for −40 mV are colored red; dashed lines indicate zero current levels. (C) G-V curves (squares) and h-infinity curves (circles) of K_V1.3 channels in T cells before (black) and after (red) SMase D treatment; error bars represent means ± SEM (n = 5). The curves are fits of Boltzmann functions, yielding: V_1/2 = −38.8 ± 0.2 mV and Z = 7.3 ± 0.4 (black circles); V_1/2 = −54.4 ± 0.2 mV, Z = 6.3 ± 0.2, and c = 0.04 ± 0.01 (red circles); V_1/2 = −26.1 ± 0.4 mV and Z = 3.7 ± 0.2 (black squares); and V_1/2 = −42.4 ± 0.6 mV and Z = 4.0 ± 0.3 (red squares).