Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 27;212(8):1153–1169. doi: 10.1084/jem.20142047

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Derivation of CD11c⁺ myeloid cells after immunotherapy. (A) Representative flow cytometric plots show the gating strategy used to identify CD11c⁺ Thy1.2⁻ myeloid cells in the brain and meninges of naive, carrier, and immunotherapy mice (n = 3 mice per group). CNS-infiltrating leukocytes were analyzed for expression of CD45.2, CD11b, and Ly6C. Resident microglia (gate 1) were identified as CD45^lo Ly6C⁻, whereas peripherally derived myeloid cells (gate 2) were CD45^hi Ly6C^+/−. (B and C) Pie graphs depict the percentage of CD11c⁺ cells in the brain (B) and meninges (C) determined to be either microglia or peripherally derived myeloid cells. The frequencies of CD11c⁺ microglia (mean ± SD) are provided in B. Data are representative of two independent experiments.