Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 27;212(8):1219–1237. doi: 10.1084/jem.20141254

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Kang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — IEC-specific PLD1 overexpression accelerates tumorigenesis in Apc^Min/+ mice. (A) PLD1 expression in various tissues from IEC-specific PLD1^Tg mice. Results are representative of at least four independent experiments. (B) q-RT-PCR analysis in SI from WT and Pld1^Tg mice. n = 5 per group. (C and D) WT (n = 5) and Pld1^Tg (n = 6) mice were sacrificed at 12 weeks for histology analysis. H&E staining (C) and IHC for PLD1 and β-catenin (D). (E) The histology of representative tumors (left) and incidence of tumor formation (right; n = 7 per group). (B–D) A Student’s t test was used. (F) Size distribution of polyps in the SI of the indicated mice. n = 7 per group. ANOVA F-test was used. (G and H) IHC for β-catenin (G) and Ki67 (H). Representative images were selected from at least three different fields. The expressions of nuclear β-catenin (G) and Ki67 (H) were quantified in size distribution of polyps. n = 5 per group; 10 tumors per mouse. A Chi-square test was used. (I) IB analysis from tissues of the indicated mice. (J) q-RT-PCR analysis from the indicated mice. n = 3–7 per group. (K and L) Total PA levels (K) and caspase-3 activities (L) in lysates from tumor tissues of Apc^Min/+ (n = 7), Apc^Min/+Pld1^Tg (n = 5), Apc^Min/+Pld1^−/− (n = 9), and Apc^Min/+Pld1^−/−Pld1^Tg (n = 12) mice sacrificed at 18 wk. Results are representative of three independent experiments. (M) Effect of PLD1 and/or Wnt3a (mimics; LiCl) on TCF trans-activation and cyclin D1 promoter activity. (J–M) A Student’s t test was used. All data are shown as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n.s., not significant; MW, molecular weight; a.u., arbitrary units. Bars, 100 µm.