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. 2015 Jul 27;212(8):1303–1321. doi: 10.1084/jem.20150329

Figure 2.

Figure 2.

MHC class I–dependent alloantigen and third party antigen presentation in the mLNs. (a and b) Lethally irradiated female or male BALB/c recipients (CD45.1+CD45.2−) were transplanted with BM and T cells from female B6 (CD45.1+CD45.2−) mice. On day 12 female CFSE-labeled HY-TCR CD8 Tg cells (CD45.1−CD45.2+) were injected, and 4 d later spleen, mLNs, and iLNs were isolated. Representative CFSE dilution plots (a) and absolute numbers of HY-TCR CD8 Tg cells detected as Vβ8+CD8+CD45.2+ cells (b). Data shown (n = 5–6 per group) are representative of two replicate experiments. **, P = 0.0043; *, P = 0.0209. (c and d) Lethally irradiated BALB/c recipients were transplanted with BM from B6.WT or B6.CD11c.ova mice and B6.WT T cells. On day 12 CFSE-labeled OT-II cells were injected, and 3 d later spleen, mLNs, and iLNs were isolated. Representative CFSE dilution plots (c) and absolute numbers of OT-II cells detected as Vβ5+Vα2+CD4+ cells (d). Data shown are combined from two replicate experiments (n = 11–12 per group). ***, P = 0.0003. Data are represented as mean ± SEM.