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. 2015 Jul 27;212(8):1303–1321. doi: 10.1084/jem.20150329

Figure 7.

Figure 7.

Donor-derived IL-12 and IL-6 drive T cell expansion and differentiation in the mLNs. BALB/c mice were transplanted with TCD BM from B6.WT or B6.IL-12p40−/− mice (a–e) or B6.IL-6−/− mice (g–k) with B6.WT T cells. TEaluc+ cells were injected on day 12, and readouts were measured 3 d later in mLNs. (a and g) Representative BLI pictures (left) and ratio of BLI signals from B6.WT BM recipients to those from B6.IL-12p40−/− BM recipients (a) or B6.IL-6−/− BM recipients (g) by mixed-model analysis (right). ***, P = 0.00071 (a); ****, P < 0.0001 (g) mLN versus whole body. Data shown are combined from two replicate experiments (n = 12 [a] or 11 [g] per group). (b, c, h, and i) Representative FACS plots (b and h) and quantification (c and i) of IFN-γ, IL-17A, and Foxp3 expression gated on TEa cells. **, P = 0.0020 for IFN-γ (c); *, P = 0.0396 for IL-17A (i). Data shown are combined from two replicate experiments (n = 11–12 [b and c] or 8 [h and i] per group). (d, e, j, and k) Representative FACS plots (d and j) and quantification (e and k) of absolute number of DCs and their YAe expression. Data shown are combined from two replicate experiments (n = 7–9 [d and e] or 10–11 [j and k] per group). Data are represented as mean ± SEM. (f) BALB/c mice were transplanted with TCD BM and T cells from B6.CD45.1+ mice, and DC subsets were isolated by FACS sorting of mLNs combined from five mice on day 12. IL-6 quantitative RT-PCR was performed. Data are shown from two separate experiments, each combining mLNs from five mice. Means are shown.