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. 2015 Jul 27;212(8):1185–1202. doi: 10.1084/jem.20141286

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Figure 9. — Antioxidant treatment rescues myeloid differentiation of human AK2^R175Q/R175Q iPSCs. (A) Microscopy analysis of in vitro myeloid maturation of AK2^R175Q/R175Q iPSCs in the presence of different concentrations of the antioxidant agent GSH in the culture medium as indicated. Data are representative of at least three independent experiments. (B) Maturation stage–specific comparison of GSH-treated and untreated AK2^R175Q/R175Q myeloid lineage cells versus control. Data are presented as the percent fraction of total myeloid cells and represent the mean of three experiments. Error bars depict standard error (***, P < 0.0001, χ² test). iPSCs generated from a human foreskin fibroblast line were used as WT control. (C) Microscopy analysis of in vitro myeloid maturation of AK2^R175Q/R175Q iPSCs in the presence of 25 ng/ml G-CSF, 1 µM ATRA, or 3 mM GSH. Magnified images of boxed areas are presented in the right panels. Data are representative of at least two independent experiments. Bars: (A and C, left) 100 µm; (A and C, right) 10 µm.