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. Author manuscript; available in PMC: 2015 Jul 28.
Published in final edited form as: Hum Mol Genet. 2007 Aug 28;16(22):2720–2728. doi: 10.1093/hmg/ddm226

Figure 2.

Figure 2

Mutant SOD1 disrupts FAT of MBOAPP. Rat cortical neurons were co-transfected with APP-EGFP and vector containing CAT, SOD1wt, SOD1G37R, SOD1G93A, SOD1G85R or SOD1A4V. MBOAPP FAT was recorded in time-lapse (1–5 s interval, 10 min). Representative kymographs showing transport of MBOAPP in SOD1wt and SOD1G37R transfected neurons are shown (A). Total transport was quantified as the distance moved per minute of observation time (anterograde and retrograde movements combined; Ba). The distances that MBOAPP moved anterograde (Bb) and retrograde (Bc) were also determined. Total, retrograde and anterograde MBOAPP movement were all significantly less in the presence of mutant SOD1s. Statistical significance was determined by one-way ANOVA followed by Newman–Keuls Multiple Comparison Test (ns: not significant, * P < 0.05, ** P < 0.01).