Figure 1.

Example of a complete stimulation protocol showing the force values recorded from a wild-type Soleus. The muscle is initially stimulated with four single pulses (Tw), delivered alternatively one directly and one through the nerve. Subsequently, a series of eight pulse trains are delivered to the specimen at the indicated frequency, alternatively directly and through the nerve to measure the force-frequency curves. The protocol ends up with two fatigue paradigms: the first delivered at the firing frequency (35 Hz) and the second at tetanic frequency (80 Hz). Insets show stimulation pulses and force response curves on a magnified time scale