Table 1.

DNA damage in male rats following treatment with 2-acetylaminofluorene.^a

Dose (mg/kg/day)	% Tail DNA		Tail length (μ)	OTM	# Hedgehogs
	Meana	p-value	Mean	Mean	Mean
Liver
0	0.4 ± 0.10	–	30.6 ± 0.68	0.0 ± 0.01	2.4 ± 0.60
250	1.5 ± 0.47	0.0233b	33.1 ± 2.33	0.2 ± 0.06	1.8 ± 0.73
500	0.8 ± 0.08	0.5930	32.0 ± 0.69	0.1 ± 0.01	2.2 ± 0.58
1000	1.0 ± 0.20	0.3395	32.2 ± 1.46	0.1 ± 0.02	1.0 ± 0.45
Trend test p-value	p = 0.3036
EMS	14.8 ± 1.44	0.0003c	51.6 ± 2.17	2.4 ± 0.26	2.8 ± 0.58
Stomach
0	13.6 ± 1.66	–	45.3 ± 0.84	2.1 ± 0.29	36.8 ± 12.07
250	18.8 ± 2.68	0.1860	46.0 ± 1.66	2.9 ± 0.49	60.2 ± 18.20
500	12.6 ± 1.60	0.9634	45.6 ± 1.85	1.9 ± 0.27	40.6 ± 6.96
1000	10.8 ± 1.63	0.6076	41.7 ± 0.92	1.5 ± 0.23	25.6 ± 7.05
Trend test p-value	p = 0.7083
EMS	31.9 ± 2.21	<0.0001c	53.6 ± 1.99	6.3 ± 0.70	125.4 ± 22.27

Abbreviations: EMS = ethyl methanesulfonate (positive control); OTM = olive tail moment.

^aData are presented as mean ± standard error of the mean; % tail DNA, tail length and OTM means are calculated from slide median data.

^bSignificant at p < 0.05.

^cSignificant at p < 0.025.