Skip to main content
. 2015 Jun 24;7(7):2354–2370. doi: 10.3390/toxins7072354

Figure 2.

Figure 2

Immunohistochemical analysis comparing the specificity of antibodies against SNAP25 in sections of rat bladder following treatment with either onabotulinumtoxinA (10 U/kg) or vehicle. (A–E) Confocal images of bladder detrusor muscle (DM) injected with onabotulinumtoxinA and probed with (A) commercial mAb (SMI-81R) against full-length (206) and cleaved (197) SNAP25; (B) a second commercial mAb (MC-6050) against SNAP25197 & SNAP25206; (C) commercial mAb (MC-6053) against SNAP25197; (D) Ab632-rMAb against SNAP25197 and (E) RGT-1092 pAb against SNAP25197; (F–J) Confocal images of control rat bladder injected with vehicle and probed with the same five antibodies. SNAP25-IR signal is in green. Arrows ((F) and (J)) point to IR-signal within nerve fibers from vehicle treated rat bladder. DM, detrusor muscle; Scale bar = 50 µm.